Dishes

The Vitrolife Labware dishes are specifically designed and certified for IVF, enabling a safe environment for your IVF procedures.

Application : Ready to use.

Storage : Store in +2 to +30°C

Challenges with plastic disposables
Optimisation of the embryo culture system

The ability to culture viable embryos involves more than using appropriate culture media. There are many variables that can have an impact on the outcome of an IVF cycle, all of which need to be taken into account in order to optimise pregnancy rates^{1, 2}. This is particularly critical during treatment of infertility since gametes and embryos are extremely sensitive. Precautions have to be taken at every step to prevent toxic or harmful components from entering the culture system.

Plastic disposables and reprotoxicity

Plastic disposables are used throughout the IVF process, from oocyte aspiration to embryo transfer. However only a small percentage of the contact supplies and tissue cultureware used in IVF is suitably tested.

When plastic disposables are insufficiently quality controlled, they can contain components that are toxic to human reproductive cells such as gametes and embryos. This phenomenon can be referred to as reprotoxicity and is defined as a negative influence on the physiology and viability of human gametes and embryos. Reprotoxicity can result in reduced gamete and embryo viability with a subsequent reduction in implantation rate or ongoing pregnancy rates³.

Vitrolife MEA can detect sub-optimal conditions

It has been reported that not all disposables on the market used for IVF fulfil the quality standard needed for safe procedures. Approximately 25% of all contact materials failed pre-screening with an accurate and sensitive Mouse Embryo Assay (MEA) and were considered sub-optimal for IVF⁴.

Vitrolife has developed the most sensitive MEA protocols. These assays are capable of detecting toxic and sub-optimal raw materials, media, and contact materials. The MEA from Vitrolife is sensitive enough to identify subtle problems that will also lead to impaired human embryo development.

Create optimal culture conditions by understanding the importance of extensive quality testing

Plastic disposables are used throughout the IVF process, but only a small percentage of them are suitably tested. When insufficiently quality controlled, plastic disposables can contain components that are toxic to gametes and embryos. 

Reprotoxicity has a negative influence on the physiology and viability of human gametes and embryos.

Reprotoxicity can however be minimised by using media and disposables that have been accurately tested. 

Embryo safe dishes enabling control

Our dishes are specifically designed to support and facilitate the various procedures during the IVF process to make your way of working easier and more effective. Vitrolife dishes are manufactured with quality controlled and certified materials. The finished products are further tested to offer a secure environment for gametes and embryos.

 

Temperature consistency

The absolute flat bottom of the square dishes, enables full contact with the heated stage. All square dishes receive the same bottom temperature when placed on a heated stage.

Labelling area

To secure patient identification, the square dishes have a dedicated area for labels or barcodes, separated from the handling area.

Tapered edges

Tapered edges provide easier access to embryos as they are also clearly visible at the periphery of the well. All dishes except ICSI dish and Collection dish 90 mm.

Dishes for every procedure

Micro-droplet Culture Dish

This unique innovation takes culture in micro-droplets to a higher level. The dish is equipped with 12 micro-wells optimised for 25-35 µL droplets. The α-numeric identification secures and facilitates embryo identification, both when viewed in and outside of the microscope.

5 Well Culture Dish

The 5 Well Culture Dish ensures embryo viability, as each well is surrounded by either air or media, resulting in a homogenous temperature.

Centre Well Dish

The Centre Well Dish is a multi-purpose dish for fertilisation, cryo-procedures, embryo culture and embryo transfer. The oval centre well allows for easy instrument access from the sides. The small diameter of the bottom facilitates easy embryo location.

ICSI Dish

The square format and low profile with low rims allow for easy instrument access. The α-numeric identification secures and facilitates embryo identification, both when viewed in and outside of the microscope.

Culture Dish 40 mm

Like the other dishes intended for culture, this dish has tapered edges. The ramp makes for easier access to embryos as they are clearly visible when at the periphery of the wall. The dish has a security ledge. 

Round Dishes 60 and 90 mm

The Collection dish 90 mm is dedicated for oocyte retrieval, while the Culture dish 60 mm can be used for oocyte retrieval, fertilisation and culture.

Temperature consistency ensures embryo viability

Embryos in culture are very sensitive to temperature fluctuations. The square dishes have an absolute flat bottom which enables full contact with the heated stage. The picture shows four Vitrolife Labware dishes on a heated stage calibrated to 37°C.

Once you have calibrated the temperature of your heated stage you can feel confident that all dishes will have the same temperature. This optimises heat transfer, minimises variation between dishes and enables consistent temperature.

Optimise media temperatures in dishes with VitroTemp

VitroTemp™ makes it easier to maintain the optimal temperature of media in dishes based on accurate fast and simple measurements that replicate the actual conditions for procedures used in the IVF lab. It is a specially designed temperature measurement solution that combines an advanced digital readout instrument with a custom made probe, a Pt100 sensor attached to the bottom of the Vitrolife square dishes. It’s easy to use and provides more consistent temperature measurements than most thermometers commonly used in IVF labs today¹.